FIGURE 4.

Localization of integrin α₆β₄ in the hemidesmosome-like structure of Caco-2 cells. Caco-2 cells were transfected with a stealth RNAi negative control duplex or a stealth RNAi duplex against human integrin β₄. To make the cell border clear, pAcGFP1-Mem vector was co-transfected. Confluent cell monolayers of control (Control-KD-Caco-2) and integrin β₄ knockdown (Integrin β₄-KD-Caco-2) Caco-2 cells were stained with the anti-integrin β₄ and the anti-integrin α₆ Abs. Inset boxes show the area of high magnification images. Scale bars, 10 μm. A, control-KD-Caco-2 cells; B, integrin β₄-KD-Caco-2 cells. Arrowheads, hemidesmosome-like structure. C, expression level of integrin β₄ in control-KD-Caco-2 and integrin-β₄-KD-Caco-2 cells. Lysates from control-KD-Caco-2 and integrin-β₄-KD-Caco-2 cells were separated by SDS-PAGE, followed by Western blotting using the anti-integrin-β₄ and the anti-actin Abs. IB, immunoblot.