FIGURE 5.

Co-localization of Necl-2 with integrin β₄ at the basal side of the lateral plasma membrane. Immunofluorescence staining for integrin β₄ and Necl-2 in Caco-2 cells. Caco-2 cells were transfected with pAcGFP1-Mem to make the cell border clear. Confluent monolayers of Caco-2 cells were stained with the anti-integrin β₄ and the anti-Necl-2 Abs. The dashed lines in the xy sectional images correspond to the sites of the z sections. Scale bar, 10 μm.