FIGURE 7.

No effect of Necl-2 on initial Caco-2 cell attachment. Wild-type Caco-2 cells, Necl-2 knockdown Caco-2 cells (Necl-2-KD-Caco-2), and Necl-2-overexpressing Caco-2 cells (Necl-2-Caco-2) were plated onto plates coated with BSA, laminin-332, and Matrigel. Cells were allowed to attach at 37 °C for 1 h, non-attached cells were removed, and the cells were subjected to fixation. The fixed cells were stained with crystal violet. Dye was extracted by 0.1 m sodium citrate in 50% ethanol for 15 min, and absorbance at 595 nm was measured as an indicator of the numbers of the attached cells. The results are given as the means ± S.D.