FIGURE 2.

Jak2 antisense treatment inhibits activation/phosphorylation of both Src kinase and p38 MAPK in IL-13-stimulated monocytes. Monocytes were pre-treated with antisense or sense ODNs to Jak2 and Tyk2 (A and B) prior to the addition of IL-13 (2 nm) for 15 min. The cells were lysed, and 50 μg of the total cell extracts (from each sample group) were separated by 8% SDS-PAGE and immunoblotted either with a rabbit polyclonal anti-phospho-Src (Tyr-418) antibody (upper panel of A) or with anti-phospho-p38 MAPK (Thr-180/Tyr-182) antibody (upper panel of B). Blots were then reprobed with Jak2 antibody to examine the effect of antisense ODN on Jak2 expression (middle panels of A and B). The same blots were stripped and reprobed with β-tubulin antibody to assess equal loading (lower panels of A and B). The results are representative of three independent experiments.