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. 2011 Aug 30;286(42):36864–36874. doi: 10.1074/jbc.M111.276790

FIGURE 4.

FIGURE 4.

IL-1β causes increased association of ATF-2 and c-Jun with the ITGB8 core promoter through p38- and/or NFκB-dependent pathways, respectively. A, the ITGB8 core promoter, Exon 1 (Ex1), and Intron 1 are shown in schematic form, with the position of the ITGB8 genomic locus and primers used for assessment of chromatin architecture indicated. Regions P1–3 contain sequences corresponding to the ITGB8 core promoter region. B–E, shown is chromatin immunoprecipitation of ATF-2 (B and C) or c-Jun (D and E) nuclear extracts from adult lung fibroblasts harvested 15 min (B and D) or 30 min (C and E) after treatment with or without IL-1β in the presence or absence of NFκB (BAY11-7082) or pan-p38 inhibitors (BIRB-796). After reversal of cross-linking, qPCR amplifications of regions from the ITGB8 core promoter were performed using primers to the indicated genomic regions. *, p < 0.05; **, p < 0.01; ***, p < 0.001.