FIGURE 5.

The nucleosome occupancy of the ITGB8 core promoter region varies by cell type. FAIRE were used to identify the relative nucleosome enrichment of the ITGB8 core promoter region in human astrocytes (n = 2), lung fibroblasts (n = 4), and dermal fibroblasts (n = 2). Relatively protein-free sheared genomic DNA was separated from formaldehyde cross-linked DNA and detected using qPCR with primers to the indicated ITGB8 core promoter and genomic regions. Results are expressed relative to input DNA for each cell type.