FIGURE 3.

Hypoxia induces PS expression in T cells. T cells (A) and peripheral blood mononuclear cells (B) were isolated from the peripheral blood of healthy volunteers, cultured under hypoxia for 48 h, and analyzed by flow cytometry for the frequency of PS-expressing T cells (gated cells). Panels a, freshly prepared naïve T cells; panels b, cells cultured under normoxia; panels c, cells cultured under hypoxia; panel d in A, negative staining control (stained with matched isotype IgG); panel d in B, cells cultured under hypoxia in the presence of anti-HIF1α antibody (500 ng/ml). Data represent three experiments.