FIGURE 1.
Role of helix hA in degron function. Plasmid constructs expressing native eGFP or various eGFP fusion proteins were stably transfected into cell line RJK88.13 and treated with CHX. Decay of protein expression over the indicated times was monitored by Western blotting (see “Experimental Procedures” for details). Schematic diagrams of the constructs are shown, along with representative blots. The half-lives (mean ± S.D.) are shown to the far right. A, constructs encoding eGFP, fusion protein N30-hA-eGFP, or fusion protein N30-N30-eGFP; B, constructs expressing fusion protein N30-hAP40/P41-eGFP or N30-hAP34/P35-eGFP; C and D, first order decay plots of the data in A and B, respectively, from which half-lives were determined. Error bars, S.D.