FIGURE 3.

An intrinsically disordered region from the chicken fibrinogen-α polypeptide contributes to degron function. Plasmids in which an intrinsically disordered region (residues 20–38) from the chicken fibrinogen-α polypeptide was inserted in place of the hTS IDR were stably transfected into cell line RJK88.13 and treated with CHX. Decay of exogenous expression over the indicated times was monitored by Western blotting (see “Experimental Procedures” for details). Schematic diagrams of the constructs are shown, along with representative blots. The half-lives (mean ± S.D.) are shown to the far right. A, constructs encoding FIB-hTS or a P2A derivative; B, constructs expressing eGFP fusion protein FIB-hA-eGFP or FIB-eGFP; C and D, first order decay plots of the data in A and B, respectively, from which half-lives were determined. Error bars, S.D.