FIGURE 5.
Deletion of residues within the HLH motif of the lac repressor reduces degron function. A, plasmids expressing deletion mutants LACdel9–17-eGFP or LACdel9–17-hA-eGFP were stably transfected into cell line RJK88.13 and treated with CHX. Decay of eGFP concentrations over the indicated times was monitored by Western blotting (see “Experimental Procedures” for details). Schematic diagrams of the constructs are shown, along with representative blots. The half-lives (mean ± S.D.) are shown to the far right. B, first order decay plots of the data in A, from which half-lives were determined. Error bars S.D.