Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Aug 30;286(42):36652–36666. doi: 10.1074/jbc.M111.285924

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — Ecm29 inhibits RP assembly in ump1Δ mutants. A, whole cell extracts (75 μg) were subjected to 3.5% native PAGE and proteasome activity assay with LLVY-AMC. B, RP₂-CP and RP-CP species were excised as strips from the native gel as in (A) and subjected to SDS-PAGE and immunoblotting (IB). C, the native gels from A were immunoblotted for a lid subunit Rpn5 or base subunits Rpt5 and Rpt1. D, whole cell extracts (20 μg) from A were resolved by 4–12% SDS-PAGE and immunoblotted for the indicated proteins.