Bhmt−/− mice have altered activities of enzymes involved in one-carbon metabolism. CHDH, GNMT, and PEMT activities were measured in 5-week-old Bhmt+/+ (black bar) and Bhmt−/− mice (white bar) mouse liver using radiometric assays. Data are presented as mean ± S.E., n = 6–8 per group. *, p < 0.05, different from Bhmt+/+ by Student's t test.