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. 2011 Aug 30;286(42):36258–36267. doi: 10.1074/jbc.M111.265348

FIGURE 3.

FIGURE 3.

Bhmt−/− mice have fatty liver and reduced hepatic phospholipid concentrations. A, hepatic phospholipids of 5-week-old Bhmt+/+ (black bar) and Bhmt−/− (white bar) mice were analyzed by a thin layer chromatography-phosphate assay method. Data are presented as mean ± S.E., n = 5 per group. *, p < 0.01, different from Bhmt+/+ by Student's t test. B, morphology of liver from 5-week-old mice was shown by H&E staining at ×100 magnification. Scale bar = 50 μm. C, hepatic TAG of 5-week-old Bhmt+/+ (black bar) and Bhmt−/− (white bar) mice was extracted and quantitated by a colorimetric assay. Data are presented as mean ± S.E., n = 6–8 per group. *, p < 0.05, different from Bhmt+/+ by Student's t test. D, VLDL secretion rate of 5-week-old Bhmt+/+ (black square) and Bhmt−/− (white square) mice was determined by measuring the plasma TAG after lipoprotein lipase inhibitor injection. Data are presented as mean ± S.E., n = 6 per group. *, p < 0.05, different from Bhmt+/+ by Student's t test. PLs, phospholipids.