FIGURE 4.

Bhmt deletion results in liver tumors at 1 year of age. A, visible hepatic tumors in 1-year-old Bhmt^−/− male mice. The inspection of liver specimens at ×4 magnification revealed single or multiple nodules up to 8 mm in diameter. B, H&E images of tumor bearing livers at ×400 magnification, showing representative hepatocellular carcinoma and adenoma morphology. Scale bar, 50 μm. 1, regenerative hyperplastic nodule containing streams of proliferative oval and biliary cells; 2, hepatocellular adenoma (*, center of the nodule) well circumscribed with lobular structure absent, compressive borders, and eosinophilic hepatocytes; 3, hepatocellular carcinoma represented by a large mass of abnormal vacuolated hepatocytes, containing poorly demarcated areas of smaller hepatocytes with less vacuolization, amphophilic cytoplasm, and a streaming growth pattern (arrow); 4, hepatocellular carcinoma nodule of variably sized hepatocytes exhibiting cytomegaly with hyaline droplets, small hepatocytes with streaming growth pattern, oval cell proliferation, Ito cell proliferation between cords (unlabeled arrow), and infiltration of leukocytes.