TABLE 3.
Deletion of Bhmt results in altered metabolic markers
Plasma was collected from 5-week-old Bhmt+/+ and Bhmt−/− mice after 4 h fasting. Concentrations of plasma ALT, BUN, creatinine, and activities of LDH and CK were quantitated by the Animal Clinical Chemistry and Gene Expression Facility, UNC, Chapel Hill. Plasma triacylglycerol, cholesterol, HDL cholesterol, glucose, NEFA, hydroxybutyrate were measured colorimetrically. Urine specific gravity was measured by the Department of Laboratory Animal Medicine Veterinary and Technical Services Facility, UNC, Chapel Hill. Data are presented as mean ± S.E., n = 5–7 per group.
| Organ | Clinical markers | Bhmt+/+ | Bhmt−/− |
|---|---|---|---|
| Plasma | ALT (units/liter) | 20.6 ± 4.9 | 34.7 ± 4.4a |
| BUN (mg/dL) | 12.5 ± 0.8 | 13.0 ± 1.8 | |
| Creatinine (mg/dL) | <0.1 | <0.1 | |
| LDH (units/liter) | 841 ± 67 | 689 ± 80 | |
| CK (units/liter) | 741 ± 147 | 511 ± 147 | |
| Triacylglycerol (mg/dL) | 60.9 ± 7.7 | 47.2 ± 7.9 | |
| Cholesterol (mg/dL) | 57.8 ± 4.3 | 31.6 ± 4.3b | |
| HDL-Cholesterol (mg/dL) | 45.8 ± 3.2 | 26.7 ± 5.7b | |
| Glucose (mg/dL) | 292.5 ± 33.2 | 226.0 ± 15.9 | |
| NEFA (mm) | 0.44 ± 0.08 | 0.28 ± 0.07 | |
| Hydroxybutyrate (mm) | 0.39 ± 0.08 | 0.29 ± 0.03 | |
| Urine | Specific gravity (mg/dL) | 1.039 ± 0.005 | 1.020 ± 0.002b |
a p < 0.05, different from Bhmt+/+ by Student's t test.
b p < 0.01, different from Bhmt+/+ by Student's t test.