TABLE 5.
Metabolites in 1-year-old mouse tissues
Tissues were harvested from 1-year-old Bhmt+/+ and Bhmt−/− mice. Hepatic AdoMet, AdoHcy, and plasma homocysteine and cysteine were quantified by high pressure liquid chromatography. Choline metabolites were extracted and quantified by liquid chromatography-electrospray ionization-isotope dilution mass spectrometry. Data are presented as mean ± S.E., n = 8 per group.
| Organ | Metabolites | Bhmt+/+ | Bhmt−/− |
|---|---|---|---|
| Liver | AdoMet (nmol/g tissue) | 113.1 ± 14.7 | 56.8 ± 3.9a |
| AdoHcy (nmol/g tissue) | 31.9 ± 5.3 | 176.3 ± 22.1a | |
| AdoMet:AdoHcy | 4.05 ± 0.65 | 0.35 ± 0.03a | |
| Betaine (nmol/g tissue) | 1,158 ± 330 | 34,976 ± 2,700a | |
| Choline (nmol/g tissue) | 84 ± 13 | 144 ± 26 | |
| GPCho (nmol/g tissue) | 803 ± 121 | 218 ± 21a | |
| PC (nmol/g tissue) | 674 ± 90 | 243 ± 59a | |
| PtdCho (nmol/g tissue) | 17,317 ± 683 | 14,075 ± 795a | |
| Sphingomyelin (nmol/g tissue) | 1,788 ± 127 | 1,976 ± 85 | |
| Plasma | tHomocysteine (μm) | 5.5 ± 0.7 | 37.7 ± 8.6a |
| Cysteine (μm) | 150.8 ± 17.4 | 134.6 ± 23.1 | |
a p < 0.01, different from Bhmt+/+ by Student's t test.