TABLE 1.
Dissociation constants of the interactions between BRPF2 PHD1 and H3-tail peptides determined by ITC and NMR titration experiments
| Method | Protein | Peptide | KD value |
|---|---|---|---|
| μm | |||
| NMR | WTa | unH3(1–12) | 134 ± 5.6 |
| ITC | WT | unH3(1–12) | 192 ± 9.7 |
| ITC | WT | H3(1–12)K4me1 | 552 ± 72 |
| ITC | WT | H3(1–12)K4me3 | Not detected |
| ITC | WT | H3(1–12)K4ac | Not detected |
| ITC | WT | H3(1–12)K9ac | 252 ± 41 |
| ITC | WT | H3(1–12)R2me2a | 694 ± 100 |
| ITC | D212A | unH3(1–12) | 362 ± 84 |
| ITC | D214A | unH3(1–12) | 300 ± 19 |
| ITC | D212A/D214A | unH3(1–12) | Not detected |
| ITC | N229A | unH3(1–12) | Not detected |
| ITC | D235A | unH3(1–12) | Not detected |
| NMR | WT | unH3(1–7) | 176 |
| NMR | WT | H3(1–7)T3ph | Not detected |
| NMR | WT | H3(1–7)T6ph | 1241 |
| ITC | WT | H3(30–44)K36me0 | Not detected |
| ITC | WT | H3(30–44)K36me3 | Not detected |
| NMR | WT | H3(30–44)K36me3 | Not detected |
a This wild-type protein harbors a C218S/ C225S mutation.