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. 2011 Aug 16;286(42):36907–36920. doi: 10.1074/jbc.M111.225052

FIGURE 4.

FIGURE 4.

ForC GBD lipid interactions as monitored by NMR and CD spectroscopy. A–E, 1H-15N HSQC spectra of 15N-ForC GBD WT or Δ12–23 in the presence of increasing amounts of the lipids indicated. The color coding of the spectra and the respective lipid concentrations are indicated below each plot. A, WT with 0.5–50 mm DPC; B, Δ12–23 with 0.5–50 mm DPC; C, WT with 0.48 mm PIP345 and 2–50 mm DPC; D, WT with 0.53 mm PIP45 and 2–50 mm DPC; E, WT in the presence of different bicelles (all cL ∼10%, q = 0.17–0.18, DMPC/DMPG = 4:1); F, CD spectra of ForC GBD WT and Δ12–23 as well as of a peptide corresponding to the β1-β2 loop (C-ForC5–28-C; p_ox, oxidized form; p_red, reduced form) in the absence and presence of differently composed micelles (see legend to the right) at similar concentrations as at the final point of the NMR titration.