TABLE 1.
Statistics for the 20 final water shell-refined structures of D. discoideum ForC 1–100 (ForC GBD), Protein Data Bank entry 2l1a
None of the structures had distance restraint violations of >0.5 Å or dihedral angle violations of >5°.
| Parameter | Value |
|---|---|
| Distance restraints (assigned + ambiguous) | |
| Total | 5258 (4421 + 837) |
| 15N NOESY | 1375 (1119 + 256) |
| Aliphatic 13C NOESY | 3100 (2638 + 462) |
| Aliphatic 13C NOESY (100% D2O) | 459 (408 + 51) |
| Aromatic 13C NOESY | 324 (256 + 68) |
| Intraresidue/sequential/medium range/long range | 1802/979/545/1095 |
| Hydrogen bond | 34 |
| φ/ψ/χ1 angle restraints | 42/42/61 |
| N-HN, Cα-Hα, Cα-Cβ RDCs | 71/56/24 |
| r.m.s. deviations from experimental restraints | |
| Distance (Å) | 0.0188 ± 0.0006 |
| Dihedral angle (degrees) | 0.130 ± 0.064 |
| Residual dipolar couplings (Hz) | 0.321 ± 0.006 |
| r.m.s. deviations from idealized geometry | |
| Bonds | 0.0147 ± 0.0003 |
| Angles | 1.178 ± 0.043 |
| Improper | 1.854 ± 0.074 |
| Total energy (kcal mol−1) | −3350 ± 89 |
| Procheck (72) statisticsa (%) | |
| Residues in most favored regions | 92.0 |
| Residues in additional allowed regions | 6.8 |
| Residues in generously allowed regions | 1.2 |
| Residues in disallowed regions | 0 |
| Average r.m.s. deviation to mean structure (Å) | |
| Residues 1–8, 26–93 (backbone/heavy) | 0.20/0.61 |
a For the well structured region (residues 1–8 and 26–93).