FIGURE 1.

p73 and PTEN induction in response to DNA damage. A, H1299 were transfected with human PTEN promoter coupled to the luciferase gene RSV-β-gal (control), p73α, or p73α-DDA. Each sample was done in triplicate and expressed as relative luciferase units (RLU). B, BJ-ERT cells were treated with 2 μm doxorubicin for the indicated times, and whole cell lysates were prepared for Western blotting. Blots were probed separately with polyclonal antibodies to p73 and PTEN and monoclonal to GAPDH for protein loading (bottom panels) or p53 (top panel). C, BJ-ERT (shp53) cells were treated and probed with antibodies as in B. D, H1299 cells were treated and probed with antibodies as in C.