Fig. 2.

Comparison of the capacities of PLRP2 and PTL to hydrolyze monoglycerides. Increasing concentration of PLRP2 (A) and PTL (B) was incubated with a triolein/gum arabic emulsion as a substrate, at pH 7.4, with 0.1 µg colipase and 2 mM bile salt mixture for 2 h at 37°C. Lipids were extracted and separated by TLC and quantified as described in Materials and Methods. The amount of lipid in each spot was calculated as a density proportion of the sum of all spots in a given lane, set to 100%.