Fig. 4.

Effect of bile salt concentration on hydrolysis of triglycerides and subsequent uptake of lipolysis products and reesterification with increasing concentrations of PLRP2. Caco-2 cells were incubated for 2 h with ³H-triolein/gum arabic as a substrate, at pH 7.4, with 0.1 µg colipase, 2 mM (A, C) or 4 mM (B, D) bile salt, and increasing concentrations of PLRP2 for 2 h at 37°C. TG and FFA were separated from the apical (A, B) and cellular (C, D) compartments, respectively. Data are expressed as the percent radioactivity in each lipid class after 2 h incubation. The sum of radioactivity in the apical, intracellular, and basolateral compartments corresponds to 100%.