Fig. 5.

Synergistic effect of BSSL and PLRP2 on triglyceride hydrolysis and subsequent cellular uptake and reesterification. Caco-2 cells were incubated for 2 h with ³H-triolein/gum arabic as a substrate, at pH 7.4, with 0.1 µg colipase, 4 mM bile salt mixture, and BSSL (0.5 µg) or PLRP2 (0.5 µg), separately, or 0.5 µg BSSL + 0.5 µg PLRP2 as described in Material and Methods. TG and FFA were separated and quantified in the cellular compartment (A) and apical compartment (B). Data are expressed as the percent radioactivity in each lipid class after 2 h incubation. The sum of radioactivity in the apical, intracellular, and basolateral compartments corresponds to 100%.