Fig. 7.

BSSL and PLRP2 catalyzed hydrolysis of cholesteryl esters and subsequent cellular uptake and reesterification. Caco-2 cells were incubated for 2 h with emulsified TG/cholesteryl ester as a substrate, at pH 7.4, with 0.1 µg colipase, 4 mM bile salts, and 1 µg BSSL, 1 µg PLRP2, or 1 µg BSSL + 1 µg PLRP2 as described in Materials and Methods. TG and FFA were separated and quantified in the cellular compartment (A) and in the apical compartment (B). Data are expressed as the percent radioactivity in each lipid class after 2 h incubation. The sum of radioactivity in the apical, intracellular, and basolateral compartments corresponds to 100%.