Fig. 4.

Comparison of PA (A) and LPA (B) fatty acid signatures after 30 s or 5 min of hyperosmotic stress. Lipids were isolated from Chlamydomonas after challenge with 300 mM NaCl for the respective times. Increments in 18:1n-7 and 18:3n-3, characteristic fatty acids of PPIs, were registered in both lipids after 30 s and 5 min (C). The fatty acid spectra of newly formed PA and LPA after 5 min of salt treatment (D) show LPA's significant enrichment in 18:3n-3 and 18:2, and a decrease in 18:1n-9 (mean ± SD, n = 3; *P < 0.003).