Fig. 3.

EGFP-D4 labeling of CHO cells treated with lovastatin and U18666A. A: CHO cells were treated with or without 8 μM lovastatin and 100 μM mevalonate, or 2 μg/ml U18666A in FBS- or LPDS-containing medium for 18 h. Cells were labeled with EGFP-D4 and Hoechst 33342 as described in MATERIALS AND METHODS. Images were acquired using In Cell Analyzer 1000 with a 20× objective. Bar = 20 μm. B: Fluorescence intensity of EGFP-D4 bound to cells was normalized by cell number as described in MATERIALS AND METHODS. Data are the mean ± average deviation of two independent experiments.