Table 1.
PCR primers and targeted fragments of the zds gene in C. protothecoides CS-41.
| Primers | Primer sequence (5′-3′) | Product size (bp) |
|---|---|---|
| Core fragment | 413 | |
| ZDSF | TCTTTGGCTGCTATTTCAACCTG | |
| ZDSR | ATCGTCACAGTTCAGGAAGC | |
| 5′-RACE | 691 | |
| ZRO1 | TTCAGGAAGCCCAGAGCGTAG | |
| ZRI1 | TTGCCGCCGAGGAAGAAGC | |
| 3′-RACE | 1390 | |
| ZFO1 | ACCTGCTGCTCAAGGACCAC | |
| ZFI1 | AGACGTGCGTGAGCTGGACTTC | |
| ZFI2 | CCGATTGCTGACTACATCACTGCCA | |
| ZDS-ORF | 1755 for cDNA; 4587 for DNA | |
| ZDSF1 | ATGCAGGCATCAGCGGTGGCC | |
| ZDSR1 | TCACGCAGCCACCGCCTGCT | |
| qRT-PCR | 171 | |
| ZDSRTF | TGGGCACCTCTCCCGTTG | |
| ZDSRTR | AGTTCAGGAAGCCCAGAGCG | |
| Chlorella β-actin | 166 | |
| c-actinF | TGATGAAGATCCTCACAGAGC | |
| c-actinR | TCTGGCAGTTCAAAGGTCTT |