Table 1.
Crystallographic Statistics
| Crystal | Smu wild type | Smu (U25C) |
|---|---|---|
| Data Collection | ||
| Space Group | P 21 21 21 | P 21 21 21 |
| Cell dimensions | ||
| a, b, c (Å) | 27.6, 68.2, 157.6 | 27.5, 68.6, 158.1 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 90 |
| Resolution (Å) | 52.5-2.27 (2.33-2.27) | 62.9-1.95 (2.01-1.95) |
| Rp.i.m. | 0.038 (0.493) | 0.025 (0.431) |
| Mn (I/σI) | 14.3 (2.0) | 20.3 (2.5) |
| Completeness (%) | 96.4 (98.9) | 98.4 (97.1) |
| Redundancy | 4.1 (3.6) | 3.5 (3.7) |
| Wavelength (Å) | 1.1051 | 1.1051 |
| Phasing | ||
| Number of heavy atoms | 5 | 5 |
| Figure of merit (before DM) | 0.34 | 0.36 |
| Refinement | ||
| Resolution (Å) | 28.6-2.27 | 62.9-1.96 |
| No. Reflections | 23076 | 22038 |
| Rwork/Rfree | 23.4/27.2 | 22.8/24.4 |
| No. Atoms | ||
| RNA | 1913 | 1913 |
| Ion | 49 | 35 |
| Ligand | 50 | 22 |
| Water | 40 | 103 |
| B-factors | ||
| RNA | 33.6 | 39.5 |
| Ion | 40.8 | 93.4 |
| Ligand | 31.9 | 41.0 |
| Water | 29.0 | 35.4 |
| RMS deviations | ||
| Bond lengths (Å) | 0.002 | 0.002 |
| Bond angles (°) | 0.513 | 0.502 |
| PDB accession code | 3SD1 | 3SD3 |