Figure 3.

Anchorage-dependent and -independent growth of HTK1-K4DT-DNp53-EGFR^WT (or EGFR^d746-750)-MYC^T58A cells. (A) HTK1-K4DT-DNp53 cells were serially infected with retroviruses expressing EGFR^WT, EGFR^d746-750, MYC^T58A or empty vectors (-). After selection, cells were harvested and subjected to SDS-PAGE. Western blots show expression of transgenes. EGFR^d746-750; constitutively active mutant of EGFR. Cells were first starved in medium without bovine pituitary extract and EGF for 72 hours and some of them were stimulated with EGF for 30 min before harvesting as indicated. HSC2; human OSCC cell line with EGFR amplification. (B) Growth curves of HTK1-K4DT-DNp53 cells expressing different transgenes as described in (A). Cells were grown as described in Fig 2(B). (C) Anchorage-independent growth of HTK1-K4DT-DNp53 cells expressing different transgenes performed as for Fig. 2(C). Scale bars, 250 μn.