Figure 4.

Anchorage-dependent and -independent growth of HTK1-E6E7-EGFR^WT (or EGFR^d746-750)-MYC^T58A cells. (A) HTK1-E6E7 cells were serially infected with retroviruses expressing EGFR^WT, EGFR^d746-750, MYC^T58A or empty vectors (-). After selection, cells were harvested and subjected to SDS-PAGE. Western blots show expression of transgenes. HSC2; human OSCC cell line with EGFR amplification. (B) Growth curves of HTK1-E6E7 cells expressing different transgenes as described in (A). Cells were grown as described in Fig 2(B). (C) Anchorage-independent growth of HTK1-K4DT-E6E7 cells expressing different transgenes performed as for Fig. 2(C). Scale bars, 250 μm.