Figure 4.

ZEB1 and β-catenin colocalize at the nucleus of invasive cancer cells at the tumor front of colorectal carcinomas. While in epithelial cells of well-differentiated areas of colorectal carcinomas β-catenin has a membranous/ cytoplasmic distribution it becomes mostly nuclear in these invasive cancer cells. ZEB1 (green, E-20 antibody, Santa Cruz Biotechnology) and β-catenin (red, Ab6032 antibody, Abcam) colocalize (yellow, merge panel) in invasive cancer cells of a sporadic colorectal carcinoma [35,78]. Labeling with 4',6-diamidino-2-phenylindole (DAPI) is also shown. Scale bar represents 25 μm.