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. 1981 Jun;78(6):3678–3682. doi: 10.1073/pnas.78.6.3678

Crystalline arrays of membrane-bound acetylcholine receptor.

J Kistler, R M Stroud
PMCID: PMC319634  PMID: 6943572

Abstract

Electron micrographs of tubular structures with a crystalline arrangement of membrane-bound acetylcholine receptor oligomers have been analyzed by digital image reconstruction. The receptor molecules are oriented synaptic side out, and in projection they appear to be asymmetric and have a defined orientation. All four subunits are contained in the oligomers as demonstrated by immunoelectron microscopy; these structures therefore appear to be suitable for subunit localization in the oligomer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aebi U., Bijlenga R. K., ten Heggeler B., Kistler J., Steven A. C., Smith P. R. Comparison of the structural and chemical composition of giant T-even phage heads. J Supramol Struct. 1976;5(4):475–495. doi: 10.1002/jss.400050406. [DOI] [PubMed] [Google Scholar]
  2. Cartaud J., Benedetti E. L. A morphological study of the cholinergic receptor protein from Torpedo marmorata in its membrane environment and in its detergent-extracted purified form. J Cell Sci. 1978 Feb;29:313–337. doi: 10.1242/jcs.29.1.313. [DOI] [PubMed] [Google Scholar]
  3. Cartaud J., Benedetti E. L., Cohen J. B., Meunier J. C., Changeux J. P. Presence of a lattice structure in membrane fragments rich in nicotinic receptor protein from the electric organ of Torpedo marmorata. FEBS Lett. 1973 Jun 15;33(1):109–113. doi: 10.1016/0014-5793(73)80171-1. [DOI] [PubMed] [Google Scholar]
  4. Claudio T., Raftery M. A. Immunological comparison of acetylcholine receptors and their subunits from species of electric ray. Arch Biochem Biophys. 1977 Jun;181(2):484–489. doi: 10.1016/0003-9861(77)90254-5. [DOI] [PubMed] [Google Scholar]
  5. Hamilton S. L., McLaughlin M., Karlin A. Formation of disulfide-linked oligomers of acetylcholine receptor in membrane from torpedo electric tissue. Biochemistry. 1979 Jan 9;18(1):155–163. doi: 10.1021/bi00568a024. [DOI] [PubMed] [Google Scholar]
  6. Kistler J., Aebi U., Onorato L., ten Heggeler B., Showe M. K. Structural changes during the transformation of bacteriophage T4 polyheads: characterization of the initial and final states by freeze-drying and shadowing Fab-fragment-labelled preparations. J Mol Biol. 1978 Dec 15;126(3):571–589. doi: 10.1016/0022-2836(78)90059-1. [DOI] [PubMed] [Google Scholar]
  7. Klymkowsky M. W., Heuser J. E., Stroud R. M. Protease effects on the structure of acetylcholine receptor membranes from Torpedo californica. J Cell Biol. 1980 Jun;85(3):823–838. doi: 10.1083/jcb.85.3.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klymkowsky M. W., Stroud R. M. Immunospecific identification and three-dimensional structure of a membrane-bound acetylcholine receptor from Torpedo californica. J Mol Biol. 1979 Mar 5;128(3):319–334. doi: 10.1016/0022-2836(79)90091-3. [DOI] [PubMed] [Google Scholar]
  9. Lindstrom J., Merlie J., Yogeeswaran G. Biochemical properties of acteylcholine receptor subunits from Torpedo californica. Biochemistry. 1979 Oct 16;18(21):4465–4470. doi: 10.1021/bi00588a003. [DOI] [PubMed] [Google Scholar]
  10. Moody M. F. Application of optical diffraction to helical structures in the bacteriophage tail. Philos Trans R Soc Lond B Biol Sci. 1971 May 27;261(837):181–195. doi: 10.1098/rstb.1971.0049. [DOI] [PubMed] [Google Scholar]
  11. Moore W. M., Holladay L. A., Puett D., Brady R. N. On the conformation of the acetylcholine receptor protein from Torpedo nobiliana. FEBS Lett. 1974 Sep 1;45(1):145–149. doi: 10.1016/0014-5793(74)80832-x. [DOI] [PubMed] [Google Scholar]
  12. Nickel E., Potter L. T. Ultrastructure of isolated membranes of Torpedo electric tissue. Brain Res. 1973 Jul 27;57(2):508–517. doi: 10.1016/0006-8993(73)90158-3. [DOI] [PubMed] [Google Scholar]
  13. Raftery M. A., Hunkapiller M. W., Strader C. D., Hood L. E. Acetylcholine receptor: complex of homologous subunits. Science. 1980 Jun 27;208(4451):1454–1456. doi: 10.1126/science.7384786. [DOI] [PubMed] [Google Scholar]
  14. Reynolds J. A., Karlin A. Molecular weight in detergent solution of acetylcholine receptor from Torpedo californica. Biochemistry. 1978 May 30;17(11):2035–2038. doi: 10.1021/bi00604a001. [DOI] [PubMed] [Google Scholar]
  15. Ross M. J., Klymkowsky M. W., Agard D. A., Stroud R. M. Structural studies of a membrane-bound acetylcholine receptor from Torpedo californica. J Mol Biol. 1977 Nov;116(4):635–659. doi: 10.1016/0022-2836(77)90264-9. [DOI] [PubMed] [Google Scholar]
  16. Schiebler W., Hucho F. Membranes rich in acetylcholine receptor: characterization and reconstitution to excitable membranes from exogenous lipids. Eur J Biochem. 1978 Apr;85(1):55–63. doi: 10.1111/j.1432-1033.1978.tb12211.x. [DOI] [PubMed] [Google Scholar]
  17. Strader C. B., Revel J. P., Raftery M. A. Demonstration of the transmembrane nature of the acetylcholine receptor by labeling with anti-receptor antibodies. J Cell Biol. 1979 Nov;83(2 Pt 1):499–510. doi: 10.1083/jcb.83.2.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Strader C. D., Raftery M. A. Topographic studies of Torpedo acetylcholine receptor subunits as a transmembrane complex. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5807–5811. doi: 10.1073/pnas.77.10.5807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stroud R. M., Agard D. A. Structure determination of asymmetric membrane profiles using an iterative Fourier method. Biophys J. 1979 Mar;25(3):495–512. doi: 10.1016/S0006-3495(79)85319-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wise D. S., Schoenborn B. P., Karlin A. Structure of acetylcholine receptor dimer determined by neutron scattering and electron microscopy. J Biol Chem. 1981 Apr 25;256(8):4124–4126. [PubMed] [Google Scholar]
  21. Zingsheim H. P., Neugebauer D. C., Barrantes F. J., Frank J. Structural details of membrane-bound acetylcholine receptor from Tropedo marmorata. Proc Natl Acad Sci U S A. 1980 Feb;77(2):952–956. doi: 10.1073/pnas.77.2.952. [DOI] [PMC free article] [PubMed] [Google Scholar]

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