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Analysis of the expression of selected collagen and hedgehog-related genes by RT-qPCR at 21 hours (grey columns) and 24 hours (dark columns) of larval development. Mean values of quadruplicates (21 hours) and triplicates (24 hours) are expressed as the ratio of the normalized expression values relative to the ama-1 gene in RNAi inhibited cultures compared to controls. SD are indicated by vertical bars.
