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. Author manuscript; available in PMC: 2012 Oct 11.
Published in final edited form as: Curr Biol. 2011 Sep 22;21(19):1615–1623. doi: 10.1016/j.cub.2011.08.033

Figure 5. Identification of additional candidate Cln2 docking sites.

Figure 5

(A) Candidate Cln2 docking sites from seven Cdk substrates (see Figure S4) were inserted into a Ste20Ste5PM chimera lacking its endogenous docking site. These derivatives were compared to chimeras containing the Ste5 or Ste20 docking sites inserted at the same position, or no docking site (none). Cln2 inhibition of pheromone response was assayed as in Figure 4. Bars, mean ± SD (n = 5)

(B) The same candidate docking sites used in panel A were inserted at the end of a Ste5 fragment (Ste51–260) that lacks its endogenous docking site, and Cln2-driven phosphorylation was assayed. See Figure S4C for additional repetitions.