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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Cancer Res. 2011 Aug 22;71(20):6310–6319. doi: 10.1158/0008-5472.CAN-10-3397

Figure 1. DXR induces up-regulation of RGS6 in human cancer cell lines.

Figure 1

A. DXR treatment (1 μM, 24 h) induces up-regulation of endogenous RGS6 protein levels in U-87 and MCF-7 human cancer cells. A representative Western blot of three independent experiments is shown. B. DXR dose-dependently activates transcription from the RGS6 gene promoter in U-87 and MCF-7 cells. The human RGS6 promoter (bp −1450 to +52) in pGL3 basic vector was transfected into U-87 and MCF-7 cells. Thirty-six h after transfection, cells were then treated with indicated concentrations of DXR for 6 h. Luciferase activity was determined as described under “Material and Methods.” The luciferase activity of untreated (0 μM DXR) cells was set as 1. Results represent means ± S.E. of three independent experiments (*p < 0.001).