Fig. 1.

Schematic diagrams of the organization of HEV luciferase replicons and their derived HVR deletion mutants. (A) Subgenomic luciferase replicon of genotype 1 human HEV. The putative functional domains are indicated at the top. MT, methyltransferase; Y, Y domain; P, papain-like cysteine protease; H, hypervariable region; X, X domain; HEL, helicase; RdRp, RNA-dependent RNA polymerase. The various HEV HVR deletion mutants and the relative positions of the deletions are indicated schematically. (B) Subgenomic luciferase replicon of avian HEV, with the HVR (aa 557 to 603) indicated as a solid box. In-frame deletions of the HVR were generated by fusion PCR. The deleted amino acids are shown as a dashed line, and the relative deleted amino acid positions are indicated for each mutant.