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. 2011 Oct;85(19):10031–10040. doi: 10.1128/JVI.00763-11

Fig. 4.

Fig. 4.

Replication competency of chimeric hepatitis E viruses with swapped HVRs in Huh7 cells and infectivity in HepG2 cells. (A) Immunofluorescence staining of Huh7 cells transfected with capped RNA transcripts from infectious cDNA clones of wild-type HEV (genotype 1 HEV [pSKHEV2] and genotype 3 swine HEV [pSHEV3]) and their derivative chimeric viruses (pSKHEV2-Sw, pSKHEV2-Av, and pSHEV3-Hu). At 6 days posttransfection, Huh7 cells were stained by IFA for HEV ORF2 antigen (green) and were viewed by confocal microscopy. (B) Immunofluorescence staining of HepG2 cells infected with lysates of Huh7 cells transfected with RNA transcripts from the wild-type and chimeric virus clones in panel A. Lysates were collected from Huh7 cells transfected with wild-type HEVs and their derivative chimeras on day 9 posttransfection and were used to infect HepG2 cells. The HepG2 cells were stained by IFA for HEV ORF2 antigen (green) and then viewed by confocal microscopy.