Table 1.
Oligonucleotide primers used for construction of genotype 1 human HEV luciferase replicon, avian HEV luciferase replicon, HVR deletion mutants, and chimeric HEV replicons
| Primer | Sequencea (5′ → 3′) | Polarity |
|---|---|---|
| Primers for genotype 1 human HEV constructs | ||
| HuLP1 | GATTGGCATGCTACAGGCTGTTGCTGATGG | + |
| HuLP2 | GCGAATTCCTATTATTCTTGCCGCCCTTCTTGG | − |
| HuLP3 | TTTGGCGTCTTCCATGGTCGCGAACCCATG | − |
| HuLP4 | CATGGGTTCGCGACCATGGAAGATGCCAAAAAC | + |
| HuGAAF | GCTGCCTTTAAAGGTGCTGCTTCGATAGTGCT | + |
| HuGAAR | AGCACTATCGAAGCAGCACCTTTAAAGGCAGC | − |
| HuF | GGGAGCATGCTCAGAAGTTTATAACACGCC | + |
| HuR | GTACCTCTGGTAAAATGCATGACAGAGCCC | − |
| Hurr | AGCATCAACCTCCGACCAAGTGCGGGTGTA | − |
| Huf1 | TCGGAGGTTGATGCTTCTATACCTAGTAGG | + |
| Huf2 | TCGGAGGTTGATGCTCCCCCCCCTGCACCG | + |
| Huf3 | TCGGAGGTTGATGCTCTCTCTGCTCCGGCG | + |
| Huf4 | GAGCCTTCTATACCTGCTCCTGGCGCTACC | + |
| Huf5 | CAGCCCGACTTAGGTGCCCCAGCCATAACC | + |
| Huf6 | GTTCCTAGTCCAGCCCACCAGACGGCCCGG | + |
| Hur1 | AGGTATAGAAGGCTCAGATGTAAAACCTAA | − |
| Hur2 | ACCTAAGTCGGGCTGGGCTGGACTAGGAAC | − |
| Hur3 | GGCTGGACTAGGAACAGCATCAACCTCCGA | − |
| Huf7 | GAGCCGGCTCCTGGCCGGCATCGCCGCCTG | + |
| Huf8 | CCTGCACCGGATCCTCGGCATCGCCGCCTG | + |
| Huf9 | ACACCTACCCCGGCGCGGCATCGCCGCCTG | + |
| Hur4 | GCCAGGAGCCGGCTCACCACGCGCCGGAGC | − |
| Hur5 | AGGATCCGGTGCAGGGGGGGGTAGAGGGGC | − |
| Hur6 | CGCCGGGGTAGGTGTGGCGGCCCTACTAGG | − |
| HuHVRf | CGTCTCAGTCGCGGCATCGCCGCCTGCTCTTT | + |
| HuHVRr | CGTCTCACGACCAAGTGCGGGTGTAAAGTG | − |
| Primers for avian HEV constructs | ||
| Av-p1A | CCCGTTAACAATATGCCCTTGCCG | + |
| Av-p1F | CCAGGCGCCCCGTTACCAATATGCCCTTGCC | + |
| Av-p1R | GGTCCGCGGGGCAATGGTTATACGGGAACGG | − |
| Av-p2A | AGCGGAGACGGCCGGTGGTGCGCAGTT | + |
| Av-p2B | CCGGCCGTCTCCGCTGGCTTGACTCCGACGCGC | − |
| Av-p3A | CGGGGGTGCCGGTACAGGGCCAGCGGA | + |
| Av-p3B | GTACCGGCACCCCCGCCTGAGGTCAGTGAGTCC | − |
| AvFF | GGTACGAAGTCTGCAGTTAGCAAGTGG | + |
| AvR | CGGGTTAACAAGCCAGTCGGCGGCA | − |
| AvEcoRVF | GGGGATATCTGCGTGGCCGAAAACTTTG | + |
| AvSalIR | GCGGTCGACTTATTACACGGCGATCTTG | − |
| AvBsmBIf | CGTCTCAATGGAAGATGCCAAAAACA | + |
| AvBsmBIr | CGTCTCACCATCCCACCCCACTTTCCT | − |
| AvGAAF | GTTGTGTTTCAAAGGTGCTGCTAGTGTCGTTGTCTGTG | + |
| AvGAAR | CACAGACAACGACACTAGCAGCACCTTTGAAACACAAC | − |
| Primers for chimeric HEV constructs | ||
| Huf | CGTCTCATTCCCGGCATCGCCGCCTGCTCTT | + |
| Hur | CGTCTCACGACCAAGTGCGGGTGTAAAGTG | − |
| Swf | CGTCTCAGTCGACATCTGGCTTTTCTAGCG | + |
| Swr | CGTCTCAGGAAGGCGGGGGTGTTGGTGGC | − |
| Avf | CGTCTCAGTCGACTTGGTCAAACTGCGCACCACCGGCC | + |
| Avr | CGTCTCAGGAAGCGAACCTCGCGCGTCGGAGTCAA | − |
| SwSMF | AGGGGACTTTGTATACGCGTACTTGGTCAACATCTG | + |
| SwSMR | CCCGCCTTCCCGCACGCGTCGTCTCCTCTAC | − |
| HuHVRf | GGGACGCGTACTTGGTCAGAGGTTGATGCTGTTCCT | + |
| HuHVRr | CCGACGCGTGCGGGCCGTCTGGTGGGTTATGGC | − |
a
Underlined nucleotides were required for cloning or mutagenesis purposes.