Fig. 3.

Dimerization profiles of single PR mutants in the presence of DRV. (A) COS-7 cells were cotransfected with pHIV-PR_WT^CFP plus pHIV-PR_WT^YFP (shown as WT^CFP/WT^YFP) or mutated pairs such as pHIV-PR_P1A^CFP plus pHIV-PR_P1A^YFP (shown as P1A^CFP/P1A^YFP) in the absence or presence of 1 μM DRV. On day 3 after transfection, CFP^A/B ratios were determined. (B) COS7 cells were cotransfected with plasmid pair pHIV-PR_A28S^CFP and pHIV-PR_A28S^YFP in the absence or presence of an agent (1 μM GRL-0216, DRV, GRL-98065, TPV, or TMC126), and CFP^A/B ratios were determined as described above. (A) The statistical evaluation of all the changes in the CFP^A/B ratios determined in the presence or absence of DRV using the nonparametric Mann-Whitney U test, gave P values ranging 0.000037 to 0.044, except for the P value for the pair A28S^CFP and A28S^YFP, which was 0.57. (B) The differences between the CFP^A/B ratios in the absence of drug (CFP^{A/B_{No Drug}}) and the CFP^A/B ratios in the presence of 1.0 μM DRV (CFP^{A/B_{1.0 DRV}}) were statistically insignificant, indicating that all of the agents examined failed to block the dimerization of A28S^CFP/A28S^YFP.