Table 1.
HIV DRV-resistant strain HIV8MIXP51 is capable of replicating in the presence of DRVa
| Virus | DRV (μM) | Replication (ng/ml) at day postexposure: |
||||
|---|---|---|---|---|---|---|
| 1 | 3 | 5 | 7 | 9 | ||
| HIVNL4-3 (WT) | 0 | 0 | 33 ± 9.9 | 955 ± 9.9 | 993 ± 57 | 1152 ± 127 |
| 0.1 | 0 | 0 | 0 | 0 | 0 | |
| 1.0 | 0 | 0 | 0 | 0 | 0 | |
| HIV8MIXP51 | 0 | 0 | 701 ± 45 | 734 ± 68 | 771 ± 19 | 877 ± 88 |
| 0.1 | 1.5 ± 2.1 | 590 ± 103 | 682 ± 199 | 729 ± 3 | 909 ± 178 | |
| 1.0 | 0.5 ± 0.7 | 270 ± 10 | 886 ± 117 | 936 ± 18 | 1,201 ± 170 | |
a
CD4+ MT-4 cells were exposed to HIVNL4-3 or HIV8MIXP51 (a highly DRV-resistant HIV variant derived from the mixture of 8 highly-PI-resistant clinical HIV isolates exposed to increasing concentrations of DRV up to 1 μM) (24), cultured in the absence or presence of 0.1 or 1.0 μM DRV. Viral replication was monitored by the amounts of p24 Gag protein (ng/ml) produced in the culture supernatant.