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. 2011 Oct;85(19):10213–10221. doi: 10.1128/JVI.05412-11

Fig. 3.

Fig. 3.

Characterization of VP6-deficient viruses in normal BSR cells. (A) Virus growth kinetics of VP6-deficient viruses BTVE1, BTVE2, and BTVD1. Total virus titer was determined by plaque assay at 0, 2, 24, 48, and 96 h postinfection and plotted as PFU/ml in logarithmic scale. As a control, cells were infected with WT BTV. (B) Protein expression pattern in BSR cells infected with the VP6-deficient viruses BTVE1, BTVE2, and BTVD1. BSR cells were infected with viruses at an MOI of 5 and analyzed 48 h postinfection by Western blotting with specific antibodies against structural (VP1, VP4, VP5, and VP7) and nonstructural (NS1, NS2, and NS3/NS3A) proteins. Cell lysate from BSR infected with WT BTV-1 was included as a control. (C) Virus growth (PFU/ml) and protein expression (NS2) in BSR cells infected with BTVD2 are shown.