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Immune response of animals vaccinated with BTVE2 in a one- or two-dose scheme. Groups of sheep were vaccinated once with VP6-deficient virus at 1 × 108 PFU/sheep or twice at 5 × 107 PFU/sheep before challenge. (A) Seroconversion in both groups determined by competitive BTV VP7 ELISA. (B) Viremia in vaccinated and control animals determined by specific quantitative real-time RT-PCR.
