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. 2011 Oct;85(19):10167–10177. doi: 10.1128/JVI.00819-11

Fig. 2.

Fig. 2.

Comparison of genome replication, virion secretion, and protein expression/secretion among Chinese genotype C isolates. Huh7 cells were transfected with circularized HBV genome and harvested 5 days later. Clones 11.1 and 11.2 of genotype B were also transfected to serve as positive controls. (A and B) Southern blot analysis of intracellular replicative DNA and virion-associated DNA using a genotype C probe. The blots were washed at 62°C with 2× SSC-0.1% SDS solution. Virions were immunoprecipitated with an anti-HBs antibody. Lanes M, HBV DNA markers of 3.2, 1.7, and 1.5 kb at 20 pg (A) and 10 pg (B). (C) Immunoprecipitation-Western blot analysis of intracellular core protein. (D) Western blot analysis of intracellular L and S proteins and GAPDH as a loading control. (E and F) ELISA for secreted HBsAg and HBeAg (averaged from 3 transfection experiments). Core promoter mutations and the G1896A mutation are indicated above panel A and below panel F, respectively.