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Effects of probe and washing conditions on detection of Chinese genotype B and C isolates. The 3.2-kb HBV genome was released from the pUC18 vector, and 500 pg was subjected to Southern blot analysis with genotype B probe, genotype C probe, or genotype B and C probes at a 1:1 ratio. The washing conditions are indicated. The washing buffer contained 0.1% SDS, in addition to 0.5× or 2× SSC.
