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. 2011 Oct;85(19):10167–10177. doi: 10.1128/JVI.00819-11

Fig. 6.

Fig. 6.

Comparison of RNA transcription, genome replication, and protein expression/secretion between U.S. genotype B and genotype C clones. Huh7 cells were transfected with SphI dimers and harvested 5 days later. Clones 11.2W and 14.5W were derived from clones 11.2 and 14.5, respectively, by reverting the core promoter sequence. (A) Intracellular HBV RNA. (B) Replicative DNA. Lanes M, 100 pg of 3.2-kb, 1.7-kb, and 1.5-kb HBV DNA markers. (C) Immunoprecipitation-Western blot analysis of intracellular core protein. (D and E) ELISA for secreted HBsAg and HBeAg (based on 3 transfection experiments). The core promoter mutation types are as follows: 1, C1773T; 2, G1764A/C1766T/T1767G/C1773T; 3, A1752G; 4, T1754C/T1758G; 5, G1746A.