Fig. 7.

Degree of CS and level of latency in infected mice. (A) CS. CS in surviving mice was determined 30 days after ocular infection. For each bar, CS (y axis) represents the average of 40 eyes from 4 separate experiments. (B) Latency. TG from infected mice used for measuring CS were removed individually at autopsy on day 30 p.i. TaqMan PCR of total DNA isolated from TG from each mouse was performed as described in Materials and Methods using gB primers. GAPDH DNA was used to normalize the relative expression of gB DNA in TG. In each experiment, an estimated relative copy number of gB was calculated using standard curves generated from pGem-gB1. Briefly, DNA template was serially diluted 10-fold, such that 5 μl contained from 10³ to 10¹¹ copies of gB, and then subjected to TaqMan PCR with the same set of primers. By comparing the normalized threshold cycle of each sample to the threshold cycle of the standard, the copy number for each reaction was determined. Each bar represents the mean ± SEM of 40 TG.