Fig. 3.

CCR5 mediates Vγ9Vδ2 T cell migration to virus-infected cells. (A) Purified peripheral Vγ9Vδ2 T cells were assayed for their chemotactic response to the supernatants from FluA virus-infected MDMs (Materials and Methods). Background migration was measured with medium in the lower well. Supernatants were also added to both upper and lower wells as controls. (B) Vγ9Vδ2 T cells were preincubated with anti-CCR5 MAb (20 μg/ml) or isotype control mouse IgG2b (mIgG2b; 20 μg/ml) for 30 min and placed in the upper well. H1N1-infected MDM supernatant (H1-sup) or H9N2-infected MDM supernatant (H9-sup) was added (+) into the lower well. (C) Titration of anti-CCR5 MAb (1 to 20 μg/ml) effects on the migration of Vγ9Vδ2 T cells. The percentages of cells (means ± SEM) that have migrated from the upper well are shown (n = 4). *, P < 0.05; **, P < 0.01. α, anti.