Fig. 4.
Vγ9Vδ2 T cells produce differential chemokines upon human versus avian FluA virus stimulation. MDMs (105) were infected with H1N1, H9N2, or H5N1 virus at an MOI of 2 for 1 h. Then, virus-infected MDMs (vMDMs; target) were fixed with 0.1% PFA for 10 min. Purified Vγ9Vδ2 T cells were cocultured with the fixed vMDMs at an effector/target ratio of 1:1 for the indicated time. The amounts of chemokines secreted by Vγ9Vδ2 T cells were quantified by a bead-based Flow Cytomix assay (BenderMed System). Data are means ± SEM for four independent experiments. *, P < 0.05; **, P < 0.01.