Table 1.
Primer sequences used in relative quantification real-time PCR assays
| Gene | Sequence (5′→3′)a | Amplification product length (bp) |
|---|---|---|
| CCR1 | F, ACCATAGGAGGCCAACCCAAAATA | 103 |
| R, TCCATGCTGTGCCAAGAGTCA | ||
| CCR2 | F, CTACCTTCCAGTTCCTCATTTTT | 100 |
| R, ACATTTACAAGTTGCAGTTTTCAGC | ||
| CCR3 | F, TTTGTCATCATGGCGGTGTTTTTC | 169 |
| R, GGTTCATGCAGCAGTGGGAGTAG | ||
| CCR4 | F, GAGAAGAAGAACAAGGCGGTGAAGA | 200 |
| R, GGATTAAGGCAGCAGTGAACAAAAG | ||
| CCR5 | F, CAACCACAGGCAGCATTTAGCAC | 147 |
| R, GGCAGGCAGCATCTTAGTTTTTCAG | ||
| CCR6 | F, CTGCCTGAACCCTGTGCTCTACG | 171 |
| R, TTATCTGCGGTCTCACTGGTCTGC | ||
| CCR7 | F, GCCGAGACCACCACCACCTT | 105 |
| R, AGTCATTGCATCTGCTCCCTATCC | ||
| CCR10 | F, GGGCTGGAGTCTGGGAAGTGC | 183 |
| R, ACGATGACGGAGACCAAGTGTGC | ||
| CCR11 | F, TCCTCCCTGTATTCCTCACAATAG | 310 |
| R, CTGGGGACTTTAGTTACTGCCAC | ||
| CXCR1 | F, CTGAGCCCCAAGTGGAACGAGACA | 152 |
| R, GCACGGAACAGAAGCTTTATTAGGA | ||
| CXCR2 | F, CAATGAATGAATGAATGGCTAAG | 118 |
| R, AAAGTTTTCAAGGTTCGTCCGTGTT | ||
| CXCR3 | F, CCCGCAACTGGTGCCGAGAAAG | 148 |
| R, AGGCGCAAGAGCAGCATCCACAT | ||
| CXCR4 | F, ATCCCTGCCCTCCTGCTGACTATTC | 231 |
| R, GAGGGCCTTGCGCTTCTGGTG | ||
| CXCR5 | F, TCCCCTCCTCACTCCCTTCCCATAA | 224 |
| R, CCTGCGGTTCCATCTGAGTGACATC | ||
| CXCR6 | F, TTGTTTATAGCTTGCGCATTCTCAT | 189 |
| R, ATCCCCCTTGGTTTCAGCATTCTT | ||
| β-Actin | F, GGATGCAGAAGGAGATCACTG | 90 |
| R, CAAGTACTCCGTGTGGATCG |
a
F, forward; R, reverse.