Table 1.

Ability of E7 mutants with mutations in predicted surface-exposed residues to transform primary BRK cells in cooperation with activated ras, with a summary of their dimerization potential

Mutant	Mean (±SD) no. of colonies		Transformation phenotype	Mean dimerization level (±SD) in vivoa	Dimerization level in vitroa,b
	Mutant	Wild type
Y52A	9.33 ± 3.85	12.00 ± 3.00	Transforms	2.05 ± 0.06	ND
N53D	22.60 ± 17.20	26.33 ± 10.33	Transforms	1.11 ± 0.21	ND
V55T	20.00 ± 9.16	13.00 ± 3.46	Increased ability	0.00 ± 0.00	−
F57A	13.33 ± 8.08	14.00 ± 1.73	Transforms	0.24 ± 0.02	+
C59S	14.20 ± 6.68	12.00 ± 2.50	Transforms	0.92 ± 0.07	ND
K60E	19.75 ± 9.32	16.25 ± 3.40	Transforms	0.62 ± 0.01	ND
D62K	19.00 ± 9.84	13.20 ± 3.83	Transforms	2.70 ± 0.03	ND
S63D	15.25 ± 10.50	15.00 ± 4.69	Transforms	1.88 ± 0.74	ND
T64D	20.60 ± 8.32	12.00 ± 3.00	Increased ability	1.28 ± 0.24	ND
R66E	22.33 ± 6.80	12.00 ± 3.00	Increased ability	0.43 ± 0.05	+++
T72D	9.66 ± 3.51	11.66 ± 3.51	Transforms	1.05 ± 0.07	ND
H73E	14.50 ± 7.77	15.00 ± 0.00	Transforms	1.07 ± 0.02	ND
V74T	11.00 ± 1.00	12.00 ± 3.00	Transforms	0.60 ± 0.03	ND
R77E	15.00 ± 4.24	13.50 ± 2.12	Transforms	0.08 ± 0.00	+
E80K/D81K	11.33 ± 4.93	11.00 ± 3.46	Transforms	0.68 ± 0.15	ND
M84S	7.66 ± 5.50	12.00 ± 3.00	Decreased ability	0.42 ± 0.08	−
G85A	6.00 ± 4.08	10.25 ± 4.27	Transforms	0.54 ± 0.13	−
T86D	10.00 ± 4.24	13.62 ± 4.46	Transforms	0.02 ± 0.00	−
P92A	17.00 ± 6.08	11.00 ± 3.46	Increased ability	0.58 ± 0.06	ND
I93T	11.50 ± 5.00	12.75 ± 2.87	Transforms	1.37 ± 0.10	ND
QKP96-98EEA	5.25 ± 2.98	14.50 ± 4.12	Decreased ability	1.70 ± 0.05	ND
del21-24	2.66 ± 2.31	13.00 ± 3.46	Does not transform	ND	ND
C58G/C91G	4.46 ± 1.52	12.00 ± 3.00	Decreased ability	0.00 ± 0.00	−
ras only	1.50 ± 1.73	12.00 ± 3.00	Does not transform	ND	ND

^aND, not determined.

^b−, little or no dimerization; +, intermediate dimerization; +++, similar to wild type.